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KMID : 0378019680110050039
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New Medical Journal
1968 Volume.11 No. 5 p.39 ~ p.45
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Autoradiographic study on the effect of sexual hormones on DNA synthesis of the mucosae epithelial cells in mice stomach using H³-thymidine
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Abstract
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Sexually matured mice weighing an average of 20 gm. were used for this study. The male mice were divided into 3 groups: 1) normal control, 2) castrated and 3) castrated prior to administration of testosterone propionate. The female ones were divided into 4 groups: 1) diestrus, 2) estrus, 3) castrated and 4) castrated before administation of estradiol benzoate.
Sexual hormones were injected subcutaneously into the casrated animals twice at an interval of over 24 hours at . the quantity of 2 uc/g body weight. H3-thymidine of 0.8,uc/g body weight was administered intravenously to all the animals 7 hours prior to the sacrifice of the animals.
Pieces of gastric mucosa were fixed in Bouin´s fluid and made into paraffin sections 2-3 thick. SIides were prepared for autoradiography by the stripping film method and with Harris´ hematoxylin.
The results are summarized as follows:
1. Both in normal control and treated animal groups, the activity of DNA synthesis is most remarkable in the gland neck and least remarkable in the gland base.
2. In the castrated groups, the radioactive indices of gastric epithelia decreased significantly as compared with the normal groups, and the indices increased to the normal Ievel or beyond that in the event of hormonal supplement.
3. In the female radioactive indices showed a more significant increase in gastrice epithelia in diestrus than in estrus.
4. It is to be concluded that injected sexual hormones supplement hormonal deficiency in the castrated animals, and tentatively expedile the activity of DNA syntheis or cell renewal of the mouse gastic epithelia to or beyond the normal level.
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KEYWORD
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